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scaffolding [2016/02/17 14:08]
127.0.0.1 external edit
scaffolding [2016/02/23 16:16] (current)
hyjeong [Di-tag의 분리 및 quality trimming]
Line 14: Line 14:
   $ SolexaQA++ dynamictrim SB_iontor.fastq –-torrent (default: P = 0.05)   $ SolexaQA++ dynamictrim SB_iontor.fastq –-torrent (default: P = 0.05)
   $ sed -n '/​^@BYI6V.*\/​1$/​{N;​N;​N;​p;​}'​ SB_iontor.fastq.trimmed > SB-trimmed_1.fastq   $ sed -n '/​^@BYI6V.*\/​1$/​{N;​N;​N;​p;​}'​ SB_iontor.fastq.trimmed > SB-trimmed_1.fastq
-  $ sed -n '/​^@BYI6V.*\/​1$/​{N;​N;​N;​p;​}'​ SB_iontor.fastq.trimmed > SB-trimmed_1.fastq+  $ sed -n '/​^@BYI6V.*\/​2$/​{N;​N;​N;​p;​}'​ SB_iontor.fastq.trimmed > SB-trimmed_2.fastq
   [optional] SolexaQA++ lengthsort SB-trimmed_1.fastq SB-trimmed_2.fastq -l 40   [optional] SolexaQA++ lengthsort SB-trimmed_1.fastq SB-trimmed_2.fastq -l 40
  
scaffolding.txt · Last modified: 2016/02/23 16:16 by hyjeong